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from S-4 6 pages Supplement No. 12 Dated as of March 31, 2011 to the Guaranty and Collateral Agreement (The “Collateral Agreement”) Dated as of February 21, 2008, Among Radiation Therapy Services Holdings, Inc., a Delaware Corporation (“Parent”), Radiation Therapy Services, Inc., a Florida Corporation (“Borrower”), Each Subsidiary of Borrower Listed on Schedule I Thereto (Each Such Subsidiary Individually a “Subsidiary Guarantor” and Collectively, the “Subsidiary Guarantors”); the Subsidiary Guarantors and Borrower Are Referred to Collectively Herein as the “Grantors”) and Wells Fargo Bank, N.A. (As Successor to Wachovia Bank, National Association), (“Wells”), as Collateral Agent (In Such Capacity, the “Collateral Agent”)